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Monitoring Temperature Changes in Capillary Electrophoresis with Nanoliter-Volume NMR

Summary: Monitoring Temperature Changes in Capillary
Electrophoresis with Nanoliter-Volume NMR
Michael E. Lacey,, Andrew G. Webb,, and Jonathan V. Sweedler*,,
Department of Chemistry, Electrical and Computer Engineering, the Beckman Institute, University of Illinois at
UrbanasChampaign, Urbana, Illinois 61801
Nanoliter-volume proton nuclear magnetic resonance
(NMR) spectroscopy is used to monitor the electrolyte
temperature during capillary electrophoresis (CE). By
measuring the shift in the proton resonance frequency of
the water signal, the intracapillary temperature can be
recorded noninvasively with subsecond temporal resolu-
tion and spatial resolution on the order of 1 mm. Thermal
changes of more than 65 C are observed under both
equilibrium and nonequilibrium conditions for typical CE
separation conditions. Several capillary and buffer com-
binations are examined with external cooling by both
liquid and air convection. Additionally, NMR thermometry
allows nonequilibrium temperatures in analyte bands to
be monitored during a separation. As one example, a plug


Source: Andrews, Anne M. - Huck Institutes of the Life Sciences, Pennsylvania State University


Collections: Biology and Medicine