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MOLECULAR AND CELLULAR BIOLOGY, May 2005, p. 34113420 Vol. 25, No. 9 0270-7306/05/$08.00 0 doi:10.1128/MCB.25.9.34113420.2005
 

Summary: MOLECULAR AND CELLULAR BIOLOGY, May 2005, p. 34113420 Vol. 25, No. 9
0270-7306/05/$08.00 0 doi:10.1128/MCB.25.9.34113420.2005
Copyright 2005, American Society for Microbiology. All Rights Reserved.
cis-Regulatory Logic of Short-Range Transcriptional Repression in
Drosophila melanogaster
Meghana M. Kulkarni and David N. Arnosti*
Department of Biochemistry and Molecular Biology and Program in Genetics, Michigan State University,
East Lansing, Michigan
Received 29 September 2004/Returned for modification 8 December 2004/Accepted 3 February 2005
Bioinformatics analysis of transcriptional control is guided by knowledge of the characteristics of cis-
regulatory regions or enhancers. Features such as clustering of binding sites and co-occurrence of binding sites
have aided enhancer identification, but quantitative predictions of enhancer function are not yet generally
feasible. To facilitate the analysis of regulatory sequences in Drosophila melanogaster, we identified quantitative
parameters that affect the activity of short-range transcriptional repressors, proteins that play key roles in
development. In addition to the previously noted distance dependence, repression is strongly influenced by the
stoichiometry, affinity, spacing, and arrangement of activator binding sites. Repression is insensitive to the type
of activation domain, suggesting that short-range repression may primarily affect activators at the level of DNA
binding. The activity of several short-range, but not long-range, repressors is circumscribed by the same
quantitative parameters. This cis-regulatory "grammar" may aid the identification of enhancers regulated by
short-range repressors and facilitate bioinformatic prediction of the functional output of transcriptional

  

Source: Arnosti, David N. - Department of Biochemistry and Molecular Biology, Michigan State University

 

Collections: Biology and Medicine