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feature of the model and furthermore provided bio-chemical evidence for the nature of the imprint.

Summary: feature of the model and furthermore provided bio-
chemical evidence for the nature of the imprint.
Interestingly, the imprint is made only by replica-
tion of the specific strand by the lagging-strand
replication complex, supporting the `orientation of
replication model.' When the mat1 fragment, which
does not contain an origin of replication, is inverted,
imprinting is abolished. When the inverted mat1 is
facilitated to replicate in the opposite direction by
judicious placement of origins of replication or
terminators of replication next to the mat1 locus,
imprinting and switching are restored. Also, mat1
is indeed replicated in the specific direction promoting
imprinting. These results establish the orientation of
replication model and support the general model
whereby the act of DNA replication advances the
developmental program of Sc. pombe.
It is thought that during replication of the
imprinted template by the leading strand complex a
transient DSB is created. The resulting 3H


Source: Anisimova, Maria - Institute of Scientific Computing, Eidgenössische Technische Hochschule Zürich (ETHZ)


Collections: Biology and Medicine