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Vol. 151, No. 1JOURNAL OF BACTERIOLOGY, July 1982, p. 411-419 0021-9193/82/070411-09$02.00/0
 

Summary: Vol. 151, No. 1JOURNAL OF BACTERIOLOGY, July 1982, p. 411-419
0021-9193/82/070411-09$02.00/0
Structural Studies of Alfalfa Roots Infected with Nodulation
Mutants of Rhizobium meliloti
ANN M. HIRSCH,l* SHARON R. LONG,2t MISUK BANG,' NANCY HASKINS,' AND FREDERICK M.
AUSUBEL2
Department ofBiological Sciences, Wellesley College, Wellesley, Massachusetts 021811 and Department of
Cellular and Developmental Biology, Harvard University, Cambridge, Massachusetts 021382
Received 28 December 1981/Accepted 27 February 1982
Alfalfa roots infected with four nodulation defective (Nod-) mutants of
Rhizobium meliloti which were generated by transposon Tn5 mutagenesis were
examined by light and electron microscopy. In one class of Nod- mutants, which
we call nonreactive, the bacteria did not induce root hair curling or penetrate host
cells. In a second class of Nod- mutants, which we call reactive, the bacteria
induced some root hair curling and entered root epidermal cells, although no
infection threads were formed. In addition, reactive Nod- mutants induced
extensive root hair proliferation and hypertrophied roots. This study presents the
details of the phenotype of the association between each mutant strain and alfalfa
roots.
Nitrogen-fixing root nodules are produced by

  

Source: Ausubel, Frederick M. - Department of Genetics, Harvard University

 

Collections: Biology and Medicine