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DEVELOPMENTAL BIOLOGY 177, 536543 (1996) ARTICLE NO. 0183
 

Summary: DEVELOPMENTAL BIOLOGY 177, 536543 (1996)
ARTICLE NO. 0183
Developmental- and Eye-Specific Transcriptional
Control Elements in an Intronic Region
of a Ca2/
-Activated K/
Channel Gene
Robert Brenner and Nigel Atkinson
Department of Zoology, The University of Texas at Austin, Austin, Texas 78712-1064
The range of electrical properties that a neuron or muscle cell can manifest is determined by which ion channel genes it
expresses and in what amounts. The Drosophila slowpoke Ca2/
-activated K/
channel gene has four distinct promoters.
Here we assess the role that a downstream intronic region, called the C2/C3 region, plays in modulating Promoter C1 and
Promoter C2 activity. Promoter C1 and Promoter C2 appear to be responsible for all neuronal and muscle expression,
respectively. Transgenic flies were used to determine the expression pattern from each promoter in the presence and
absence of the C2/C3 region. Deletion of this region silences Promoter C1 in adult but not larval CNS and causes a
substantial reduction in Promoter C2 activity in adult but not larval muscle. The C2/C3 region also activates Promoter
C1 in the animal's eye. By placing the C2/C3 region adjacent to a basal HSP70 promoter we have demonstrated that it
contains elements that can specifically activate a heterologous promoter in the eye and in adult but not larval muscle.

  

Source: Atkinson, Nigel - Section of Neurobiology, Division of Biological Sciences, University of Texas at Austin

 

Collections: Biology and Medicine