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Proc. Nati. Acad. Sci. USA Vol. 91, pp. 3117-3121, April 1994
 

Summary: Proc. Nati. Acad. Sci. USA
Vol. 91, pp. 3117-3121, April 1994
Biochemistry
Synthesis of circular RNA in bacteria and yeast using RNA cyclase
ribozymes derived from a group I intron of phage T4
(spldng/RNA proceslg/Eschrchia coli/Saccharomyces cereviia/td gene)
ETHAN FoRD* AND MANUEL ARES, JR.t
Biology Department, Sinsheimer Laboratories, University of California, Santa Cruz, Santa Cruz, CA 95064
Communicated by Harry F. Noller, December 22, 1993
ABSTRACT Studies on the function of circular RNA and
RNA topology in vivo have been limited by the difficulty in
expressing circular RNA of desired sequence. To overcome
this, the group I intron from the phage T4 d gene was split in
a peripheral loop (L6a) and rearranged so that the 3' half
intron and 3' splice site are uptram and a 5' splice site and
5' half intron are downstream of a single exon. The group I
splicing reactions excise theinternal exon RNA asacircle (RNA
cyclase ribozyme activity). We show that foreign sequences can
be placed in the exon and made circular in vitro. Expression of
such constructs (RNA cyclase ribozymes) in Eschenchia colU

  

Source: Ares Jr., Manny - Department of Molecular, Cell, and Developmental Biology, University of California at Santa Cruz

 

Collections: Biology and Medicine