Summary: Plant Molecular Biology 39: 3544, 1999.
© 1999 Kluwer Academic Publishers. Printed in the Netherlands.
Isolation and characterization of an anther-specific gene, RA8, from rice
(Oryza sativa L.)
Jong-Seong Jeon1, Yong-Yoon Chung2, Sichul Lee1, Gi-Hwan Yi3, Byeong-Geun Oh3 and
1Department of Life Science, Pohang University of Science and Technology, Pohang, Korea 790784 (author for
correspondence); 2Department of Genetic Engineering, Paichai University, Taejon, Korea 302735; 3Yeongnam
Agricultural Experiment Station, Milyang, Korea 627130
Received 26 January 1998; accepted in revised form 10 July 1998
Key words: anther-specific, GUS, promoter, rice, tapetum, transgenic
An anther-specific cDNA clone of rice, RA8, was isolated from an anther cDNA library by differential screening.
RNA blot analysis indicated that the RA8 transcript is present specifically in anthers and the transcript level
increased as flowers matured, reaching the highest level in mature flowers. The RA8 clone contains an open reading
frame of 264 amino acid residues with a hydrophobic N-terminal region. The deduced amino acid sequences did
not show significant homology to any known sequences. Genomic DNA blot analysis showed that RA8 is a single-
copy gene. A genomic clone corresponding to the RA8 cDNA was isolated and its promoter region was fused to
the -glucuronidase (GUS) gene. Transgenic rice plants exhibited anther-specific expression of the GUS reporter