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Journal of Insect Physiology 52 (2006) 11281136 Molecular aspects of viviparous reproductive biology of the tsetse fly

Summary: Journal of Insect Physiology 52 (2006) 1128≠1136
Molecular aspects of viviparous reproductive biology of the tsetse fly
(Glossina morsitans morsitans): Regulation of yolk and milk gland
protein synthesis
Geoffrey M. Attardo, Nurper Guz, Patricia Strickler-Dinglasan1
, Serap Aksoy√
Department of Epidemiology and Public Health, Yale University School of Medicine, 60 College Street, 606 LEPH New Haven, CT 06510, USA
Received 27 June 2006; received in revised form 21 July 2006; accepted 24 July 2006
Tsetse fly (Diptera: Glossinidae) viviparous reproductive physiology remains to be explored at the molecular level. Adult females carry
their young in utero for the duration of embryonic and larval development, all the while supplying their offspring with nutrients in the
form of a ``milk'' substance secreted from a modified accessory gland. Flies give birth to fully developed third instar larvae that pupariate
shortly after birth. Here, we describe the spatial and temporal expression dynamics of two reproduction-associated genes and their
products synthesized during the first and second gonotrophic cycles. The proteins studied include a putative yolk protein, Glossina
morsitans morsitans yolk protein 1 (GmmYP1) and the major protein found in tsetse ``milk'' secretions (Glossina morsitans morsitans milk
gland protein, GmmMGP). Developmental stage and tissue-specific expression of GmmYP1 show its presence exclusively in the
reproductive tract of the fly during oogenesis, suggesting that GmmYP1 acts as a vitellogenic protein. Transcripts for GmmMGP are
present only in the milk gland tissue and increase in coordination with the process of larvigenesis. Similarly, GmmMGP can be detected
at the onset of larvigenesis in the milk gland, and is present during the full duration of pregnancy. Expression of GmmMGP is restricted
to the adult stage and is not detected in the immature developmental stages. These phenomena indicate that the protein is transferred


Source: Aksoy, Serap - School of Public Health, Yale University


Collections: Biology and Medicine