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JOURNAL OF BACTERIOLOGY, Oct. 1979, p. 246-25(0 0021-919.3/79/1 0-0246/05$02.00/0
 

Summary: JOURNAL OF BACTERIOLOGY, Oct. 1979, p. 246-25(0
0021-919.3/79/1 0-0246/05$02.00/0
Vol. 140, No. I
Cyanobacterial Ribonucleic Acid Polymerases Recognize
Lambda Promoters
STEPHAN S. MILLER, FREDERICK M. AUSUBEL, AND LAWRENCE BOGORAD)*
The Biological Laboratories, Hartard Unitersity, Coambri(ge, Massachusetts 02138
Received for publication 27 April 197.9
We compared the initiation specificities in vitro of deoxyribonucleic acid-
dependent ribonucleic acid polymerases purified from two cyanobacteria, Fre-
myella diplosiphon and Anacystis nidulans, and from Escherichia coli. A restric-
tion fragment made from lambda deoxyribonucleic acid was used as a template.
The cyanobacterial and E. coli ribonucleic acid polymerases recognized the same
lambda promoters but exhibited different sensitivities to the inhibitor heparin,
suggesting differences in the structure of the initiation complexes.
The ability of a DNA-dependent RNA polym-
erase to initiate transcription specifically at a
site or sites (promoters) on a DNA template is
defined as its initiation specificity or its selectiv-
ity (4). It is likely that RNA polymerase selec-

  

Source: Ausubel, Frederick M. - Department of Genetics, Harvard University

 

Collections: Biology and Medicine