Summary: Chemistry & Biology, Vol. 11, 309­318, March, 2004, 2004 Elsevier Science Ltd. All rights reserved. DOI 10.1016/j.chembiol.2004.02.018
Functional Evolution and Structural Conservation
in Chimeric Cytochromes P450:
Calibrating a Structure-Guided Approach
P450s in the laboratory by recombination of homolo-
gous sequences. P450s typically exhibit low sequence
identity, and annealing-based DNA-shuffling techniques
[9­13] are not useful for creating highly diverse libraries
of P450 chimeras. While several methods for making
Christopher R. Otey,1
Jonathan J. Silberg,2
Christopher A. Voigt,4
Jeffrey B. Endelman,3
Geethani Bandara,2
and Frances H. Arnold1,2,3,
*
1
Biochemistry and Molecular Biophysics Option
2
Division of Chemistry and Chemical Engineering

Source: Arnold, Frances H. - Division of Chemistry and Chemical Engineering, California Institute of Technology

Collections: Chemistry; Biology and Medicine