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Unraveling the Mechanism of a Potent Transcriptional Activator*S Received for publication, May 4, 2005
 

Summary: Unraveling the Mechanism of a Potent Transcriptional Activator*S
Received for publication, May 4, 2005
Published, JBC Papers in Press, May 10, 2005, DOI 10.1074/jbc.M504895200
Zhen Lu§, Steven P. Rowe§¶ , Brian B. Brennan§¶, Sarah E. Davis, Renee E. Metzler,
Johnathan J. Nau, Chinmay Y. Majmudar¶, Anna K. Mapp¶**, and Aseem Z. Ansari §§
From the Department of Biochemistry and The Genome Center, University of Wisconsin, Madison, Wisconsin 53706
and the ¶Department of Chemistry and Department of Medicinal Chemistry, University of Michigan,
Ann Arbor, Michigan 48109
Despite their enormous potential as novel research
tools and therapeutic agents, artificial transcription
factors (ATFs) that up-regulate transcription robustly
in vivo remain elusive. In investigating an ATF that does
function exceptionally well in vivo, we uncovered an
unexpected relationship between transcription func-
tion and a binding interaction between the activation
domain and an adjacent region of the DNA binding do-
main. Disruption of this interaction leads to complete
loss of function in vivo, even though the activation do-
main is still able to bind to its target in the transcrip-
tional machinery. We propose that this interaction par-

  

Source: Ansari, Aseem Z. - Department of Biochemistry, University of Wisconsin at Madison

 

Collections: Biology and Medicine