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Surface-Modified Hydrogels for Chemoselective Bioconjugation Kyle N. Plunkett, Aveek N. Chatterjee, N. R. Aluru,* and Jeffrey S. Moore*
 

Summary: Surface-Modified Hydrogels for Chemoselective Bioconjugation
Kyle N. Plunkett, Aveek N. Chatterjee, N. R. Aluru,* and Jeffrey S. Moore*
The Beckman Institute for Advanced Science and Technology, The University of Illinois at
Urbana-Champaign, Urbana, Illinois 61801
Received August 8, 2003; Revised Manuscript Received September 10, 2003
ABSTRACT: Patterned hydrogels of glycerol monomethacrylate (GMM)-co-acrylic acid (AA) were prepared
in microchannels using an in situ photopolymerization method. Oxidation of the hydrogel was accomplished
using aqueous sodium periodate (NaIO4) to produce an aldehyde functionalized surface. The depth of
oxidation, characterized by confocal microscopy after conjugation with a fluorescent dye, was controlled
by varying the NaIO4 concentration, reaction time, and temperature. Hydrogel oxidation was modeled
by combining the Nernst-Planck, Poisson, Arrhenius, and rate equations. The modeling studies suggest
that the glycol oxidation reaction and the diffusion of oxidant into the hydrogel are competitive factors
in determining the oxidation depth. Once modified, these hydrogels have the ability to covalently bind
small molecules and biomolecules to their surface under mild, aqueous conditions. For example,
fluorescently labeled bovine serum albumin (FITC-BSA) was site-specifically conjugated to the surface
of a photopatterned hydrogel via reductive amination.
Introduction
Surface modifications of polymeric materials have
found importance in a multitude of applications as they
provide the opportunity to transform inexpensive poly-

  

Source: Aluru, Narayana R. - Department of Mechanical and Industrial Engineering, University of Illinois at Urbana-Champaign

 

Collections: Engineering; Materials Science