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Supplementary Materials and Methods Neuronal activity was recorded from 19 male Long-Evans rats (3-5 months old, 350-450 g at

Summary: Supplementary Materials and Methods
Neuronal activity was recorded from 19 male Long-Evans rats (3-5 months old, 350-450 g at
implantation and testing) housed individually in transparent Perspex cages (54 44 35 cm
height) with water available ad libitum. The rats were kept on a 12 hr light/dark schedule and
tested in the dark phase. After surgery, the rats were placed on a food deprivation schedule that
kept the rats at ~ 90 % of their free-feeding body weight.
Electrode implantation
The rats were anesthetized (Equithesin; 1 ml/250g) and chronically implanted with one or two
microdrives each connected to 4 tetrodes of twisted 17 m HM-L coated platinum-iridium
. The electrode tips were platinum-plated before surgery in order to reduce electrode
impedances to 150-250 k at 1 kHz. During surgery, the tetrodes were inserted above the
dorsocaudal MEC, 4.5 mm lateral to the midline (ML) and 0.2-0.3 mm anterior to the transverse
sinus, at an angle of ~10 degrees in the anterior direction in the sagittal place. The depth of
implantation was always 1.5-1.8 mm. A jeweller's screw fixed to the skull served as a ground
electrode. The microdrive was secured to the skull using jewellers' screws and dental cement. A
subset of the animals received an additional set of tetrodes above the dorsocaudal MEC of the
other hemisphere (n = 5), or above CA3 (AP: -3.2 and ML: 2.8; n = 10), or above CA1 (AP: -4.0
and ML: 3.1; n = 1). In two animals, a twisted 70 m Teflon-insulated steel electrode


Source: Andrzejak, Ralph Gregor - Departament de Tecnologia, Universitat Pompeu Fabra


Collections: Computer Technologies and Information Sciences