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Vol. 171, No. 4 Identification of a Gene Linked to Rhizobium meliloti ntrA Whose
 

Summary: Vol. 171, No. 4
Identification of a Gene Linked to Rhizobium meliloti ntrA Whose
Product Is Homologous to a Family to ATP-Binding Proteins
LISA M. ALBRIGHT, CLIVE W. RONSON,t B. TRACY NIXON,: AND FRED M. AUSUBEL*
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, and Department of Molecular Biology,
Massachusetts General Hospital, Boston, Massachusetts 02114
Received 4 November 1988/Accepted 25 January 1989
The ntrA gene of Rhizobium meliloti has recently been identified and shown to be required for a diverse set
of metabolic functions (C. W. Ronson, B. T. Nixon, L. M. Albright, and F. M. Ausubel, J. Bacteriol.
169:2424-2431, 1987). As a result of sequencing the ntrA gene and its flanking regions from R. meliloti, we
identified an open reading frame directly upstream of ntrA, ORF1, whose predicted product is homologous to
a superfamily of ATP-binding proteins involved in transport, cell division, nodulation, and DNA repair. The
homology of ORF1 to this superfamily and its proximity to ntrA led us to investigate its role in symbiosis by
mutagenesis and expression studies. We were unable to isolate an insertion mutation in ORF1, suggesting that
ORF1 may code for an essential function. We identified the start of transcription for the ntrA gene in vegetative
cells and bacteroids and showed that ORF1 and ntrA are transcriptionally unlinked. ORF1 appears to be in an
operon with one or more upstream genes.
Conservation throughout a region about 250 amino acids
in length has been noted in a number of proteins involved in
transport, cell division, nodulation, and DNA repair in

  

Source: Ausubel, Frederick M. - Department of Genetics, Harvard University

 

Collections: Biology and Medicine