Summary: Current Biology, Vol. 12, 1317­1319, August 6, 2002, 2002 Elsevier Science Ltd. All rights reserved. PII S0960-9822(02)01041-2
Loss of the Putative RNA-Directed RNA Polymerase
RRF-3 Makes C. elegans Hypersensitive to RNAi
analysis of the rrf genes of C. elegans (a family of puta-
tive RNA-directed RNA polymerases [RdRP]) hinted that
mutations in the rrf-3 gene cause increased sensitivity
Femke Simmer,1
Marcel Tijsterman,1
Susan Parrish,2,3
Sandhya P. Koushika,4
Michael L. Nonet,4
Andrew Fire,3
Julie Ahringer,5
and Ronald H.A. Plasterk1,6
to RNAi [4]. To investigate this, we first assayed bacteria
expressing 80 distinct dsRNAs chosen from a genome-1
Hubrecht Laboratory
Center for Biomedical Genetics wide library designed to induce RNAi when fed to C.
elegans ([2] and R.S. Kamath et al., submitted). We pri-Uppsalalaan 8
3584 CT, Utrecht marily selected dsRNA segments that do not produce

Source: Ahringe, Julie - Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge
Chin-Sang, Ian - Department of Biology, Queen's University (Kingston)
Nonet, Michael L. - Department of Anatomy and Neurobiology, Washington University in St. Louis

Collections: Biology and Medicine