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Journal of Neuroscience Methods 94 (1999) 2740 Simultaneous multi-site recordings and iontophoretic drug and dye

Summary: Journal of Neuroscience Methods 94 (1999) 2740
Simultaneous multi-site recordings and iontophoretic drug and dye
applications along the trigeminal system of anesthetized rats
Sebastian Haidarliu *, Ronen Sosnik, Ehud Ahissar
Department of Neurobiology, The Weizmann Institute of Science, Reho6ot 76100, Israel
Received 12 June 1999; accepted 30 July 1999
A multi-electrode system that permits simultaneous recordings from multiple neurons and iontophoretic applications at two or
three different brain sites during acute experiments is described. This system consists of two or three microdrive terminals, each
of which includes four electrodes that can be moved independently and used for both extracellular recordings and microion-
tophoretic drug administration. Drug applications were performed during standard extracellular recordings of multiple single-
units via specialized combined electrodes (CEs), which enable ejection of neuroactive substances and recording of neuronal activity
from the same electrode. With this system, we were able to successfully record simultaneously from different levels (brainstem,
thalamus, and cortex) of the vibrissal ascending pathway of the anesthetized rat. Herein, examples of simultaneous recordings
from the brainstem and thalamus and from the thalamus and cortex are presented. An effect of iontophoretic applications of
agonists and antagonists of metabotropic glutamate receptors (mGluRs) in the thalamus is demonstrated, and the extent of drug
diffusion in the barrel cortex is demonstrated with biocytin. This new multi-electrode system will facilitate the study of
transformations of sensory information acquired by the whiskers into cortical representations. 1999 Elsevier Science B.V. All
rights reserved.
Keywords: Barrel cortex; Brainstem trigeminal nuclei; Microiontophoresis; Multi-electrode array; Multi-site recordings; Sensory systems;


Source: Ahissar, Ehud - Department of Neurobiology, Weizmann Institute of Science


Collections: Biology and Medicine