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nature genetics volume 31 july 2002 255 Large-scale prediction of Saccharomyces

Summary: article
nature genetics volume 31 july 2002 255
Large-scale prediction of Saccharomyces
cerevisiae gene function using
overlapping transcriptional clusters
Lani F. Wu1,3*, Timothy R. Hughes1,2*, Armaity P. Davierwala2, Mark D. Robinson2, Roland Stoughton1 &
Steven J. Altschuler1,3
*These authors contributed equally to this manuscript.
Published online: 24 June 2002, doi:10.1038/ng906
Genome sequencing has led to the discovery of tens of thousands of potential new genes. Six years after the
sequencing of the well-studied yeast Saccharomyces cerevisiae and the discovery that its genome encodes 6,000
predicted proteins, more than 2,000 have not yet been characterized experimentally, and determining their func-
tions seems far from a trivial task. One crucial constraint is the generation of useful hypotheses about protein
function. Using a new approach to interpret microarray data, we assign likely cellular functions with confidence
values to these new yeast proteins. We perform extensive genome-wide validations of our predictions and offer
visualization methods for exploration of the large numbers of functional predictions. We identify potential new
members of many existing functional categories including 285 candidate proteins involved in transcription, pro-
cessing and transport of non-coding RNA molecules. We present experimental validation confirming the involve-
ment of several of these proteins in ribosomal RNA processing. Our methodology can be applied to a variety of
genomics data types and organisms.


Source: Altschuler, Steve - Department of Pharmacology, UT Southwestern Medical Center


Collections: Biotechnology