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JOURNAL OF BACTERIOLOGY, Aug. 1995, p. 45534556 Vol. 177, No. 15 0021-9193/95/$04.00 0
 

Summary: JOURNAL OF BACTERIOLOGY, Aug. 1995, p. 45534556 Vol. 177, No. 15
0021-9193/95/$04.00 0
Copyright 1995, American Society for Microbiology
Use of Tn5tac1 To Clone a pel Gene Encoding a Highly Alkaline,
Asparagine-Rich Pectate Lyase Isozyme from an Erwinia
chrysanthemi EC16 Mutant with Deletions Affecting
the Major Pectate Lyase Isozymes
JAMES R. ALFANO, JONG HYUN HAM, AND ALAN COLLMER*
Department of Plant Pathology, Cornell University, Ithaca, New York 14853-4203
Received 20 March 1995/Accepted 22 May 1995
Erwinia chrysanthemi mutant CUCPB5047, (pelA pelE) (pelB pelC)::28bp (pelX) 4bp pehX:: Cmr
, was
constructed, mutated with Tn5tac1, and screened for isopropyl- -D-thiogalactopyranoside-dependent pectate
lyase (Pel) production. A Kmr
SacI fragment from the hyperexpressing Pel mutant CUCPB5066 was cloned
into Escherichia coli and sequenced. The gene identified, pelL, encodes a novel, asparagine-rich, highly alkaline
enzyme that is similar in primary structure to PelX and in enzymological properties to PelE.
The enterobacterium Erwinia chrysanthemi causes diseases
involving maceration and killing of parenchymatous tissues in
many plants. The bacterium also produces and secretes a va-

  

Source: Alfan, James R. - Department of Plant Pathology, University of Nebraska-Lincoln

 

Collections: Biology and Medicine