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JOURNAL OF BACTERIOLOGY, 0021-9193/01/$04.00 0 DOI: 10.1128/JB.183.15.45174525.2001
 

Summary: JOURNAL OF BACTERIOLOGY,
0021-9193/01/$04.00 0 DOI: 10.1128/JB.183.15.45174525.2001
Aug. 2001, p. 45174525 Vol. 183, No. 15
Copyright 2001, American Society for Microbiology. All Rights Reserved.
Genome Size Determination and Coding Capacity of
Sodalis glossinidius, an Enteric Symbiont of Tsetse Flies, as
Revealed by Hybridization to Escherichia coli Gene Arrays
LEYLA AKMAN, RITA V. M. RIO, CHARLES B. BEARD, AND SERAP AKSOY*
Department of Epidemiology and Public Health, Section of Vector Biology, Yale University School of Medicine,
New Haven, Connecticut 06510
Received 26 January 2001/Accepted 3 May 2001
Recent molecular characterization of various microbial genomes has revealed differences in genome size and
coding capacity between obligate symbionts and intracellular pathogens versus free-living organisms. Multiple
symbiotic microorganisms have evolved with tsetse fly, the vector of African trypanosomes, over long evolu-
tionary times. Although these symbionts are indispensable for tsetse fecundity, the biochemical and molecular
basis of their functional significance is unknown. Here, we report on the genomic aspects of the secondary
symbiont Sodalis glossinidius. The genome size of Sodalis is approximately 2 Mb. Its DNA is subject to extensive
methylation and based on some of its conserved gene sequences has an A T content of only 45%, compared
to the typically AT-rich genomes of endosymbionts. Sodalis also harbors an extrachromosomal plasmid about
134 kb in size. We used a novel approach to gain insight into Sodalis genomic contents, i.e., hybridizing its DNA

  

Source: Aksoy, Serap - School of Public Health, Yale University

 

Collections: Biology and Medicine