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Molecular Biology of the Cell Vol. 11, 287304, January 2000

Summary: Molecular Biology of the Cell
Vol. 11, 287­304, January 2000
Selective Alterations in Biosynthetic and Endocytic
Protein Traffic in Madin-Darby Canine Kidney Epithelial
Cells Expressing Mutants of the Small GTPase Rac1
Tzuu-Shuh Jou,*
Som-Ming Leung,
Linette M. Fung,* Wily G. Ruiz,
W. James Nelson,* and Gerard Apodaca§
*Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford,
California 94305-5345; and
Renal-Electrolyte Division of the Department of Medicine and Laboratory
of Epithelial Biology, University of Pittsburgh, Pittsburgh, Pennsylvania 15261
Submitted July 28, 1999; Revised September 29, 1999; Accepted October 15, 1999
Monitoring Editor: David Drubin
Madin-Darby canine kidney (MDCK) cells expressing constitutively active Rac1 (Rac1V12) accu-
mulate a large central aggregate of membranes beneath the apical membrane that contains
filamentous actin, Rac1V12, rab11, and the resident apical membrane protein GP-135. To examine
the roles of Rac1 in membrane traffic and the formation of this aggregate, we analyzed endocytic
and biosynthetic trafficking pathways in MDCK cells expressing Rac1V12 and dominant inactive


Source: Apodaca, Gerard - Departments of Medicine & Cell Biology and Physiology, University of Pittsburgh


Collections: Biology and Medicine