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Summary: SHORT COMMUNICATION
I5
M: 3D widefield light microscopy with better than
100 nm axial resolution
M. G. L. GUSTAFSSON,* D. A. AGARD* & J. W. SEDAT*
*Department of Biochemistry and Biophysics, University of California, San Francisco,
CA 94143-0448, U.S.A.
Howard Hughes Medical Institute, University of California, San Francisco, CA 94143-0448,
U.S.A.
Key words. Cytoskeleton, deconvolution, I2
M, I5
M, In
M, interference, microtubule,
optical microscopy, OTF, resolution, superresolution, widefield microscopy.
Summary
Sevenfold improved axial resolution has been achieved in
three-dimensional widefield fluorescence microscopy, using
a novel interferometric technique in which the sample is
observed and/or illuminated from both sides simultaneously
using two opposing objective lenses. Separate interference
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