Home

About

Advanced Search

Browse by Discipline

Scientific Societies

E-print Alerts

Add E-prints

E-print Network
FAQHELPSITE MAPCONTACT US


  Advanced Search  

 
Long-term Monitoring of Bacteria Undergoing Programmed Population Control in a Microchemostat
 

Summary: Long-term Monitoring of Bacteria Undergoing Programmed Population Control in
a Microchemostat
Supporting Online Material
Materials and Methods
References and Notes
Tables S1 & S2
Figures S1-S5
Materials and Methods
Microfluidic device design and fabrication: The microchemostat chip was fabricated
out of the silicone elastomer polydimethylsiloxane (PDMS) (General Electric RTV 615)
using multi-layer soft lithography, as described previously (S1). Six reactors run in
parallel on each chip.
The microchemostat reader: The reader consists of a Nikon TE 2000 (A. G. Heinze
Inc., Lake Forest, CA) inverted microscope furnished with a PRIOR Scientific XYZ
motorized stage system (A. G. Heinze Inc., Lake Forest, CA). Imaging was done using a
Plan Fluor 40X 0.75NA ph2 DLL objective. The images were captured by a charge-
coupled device (CCD) camera and digitized by a PXC200 color frame grabber
(Cyberoptics Semiconductor, Beaverton, OR). We developed Labview software to
control the synchronized operation of these components and all chip operation.
Balagaddé et al.1

  

Source: Arnold, Frances H. - Division of Chemistry and Chemical Engineering, California Institute of Technology

 

Collections: Chemistry; Biology and Medicine