Home

About

Advanced Search

Browse by Discipline

Scientific Societies

E-print Alerts

Add E-prints

E-print Network
FAQHELPSITE MAPCONTACT US


  Advanced Search  

 
A novel application of gene arrays: Escherichia coli array provides insight into the biology of the
 

Summary: A novel application of gene arrays: Escherichia coli
array provides insight into the biology of the
obligate endosymbiont of tsetse flies
Leyla Akman and Serap Aksoy*
Department of Epidemiology and Public Health, Section of Vector Biology, Yale University School of Medicine, New Haven, CT 06510
Edited by Steven E. Lindow, University of California, Berkeley, CA, and approved April 30, 2001 (received for review February 5, 2001)
Symbiotic associations with microorganisms are pivotal in many
insects. Yet, the functional roles of obligate symbionts have been
difficult to study because it has not been possible to cultivate these
organisms in vitro. The medically important tsetse fly (Diptera:
Glossinidae) relies on its obligate endosymbiont, Wigglesworthia
glossinidia, a member of the Enterobacteriaceae, closely related to
Escherichia coli, for fertility and possibly nutrition. We show here
that the intracellular Wigglesworthia has a reduced genome size
smaller than 770 kb. In an attempt to understand the composition
of its genome, we used the gene arrays developed for E. coli. We
were able to identify 650 orthologous genes in Wigglesworthia
corresponding to 85% of its genome. The arrays were also
applied for expression analysis using Wigglesworthia cDNA and 61
gene products were detected, presumably coding for some of its

  

Source: Aksoy, Serap - School of Public Health, Yale University

 

Collections: Biology and Medicine