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JOURNAL OF BACTERIOLOGY, Feb. 1990, p. 901-911 0021-9193/90/020901-11$02.00/0
 

Summary: JOURNAL OF BACTERIOLOGY, Feb. 1990, p. 901-911
0021-9193/90/020901-11$02.00/0
Copyright 1990, American Society for Microbiology
Vol. 172, No. 2
Rhizobium meliloti nodD Genes Mediate Host-Specific
Activation of nodABC
MARY A. HONMA,t MARGARET ASOMANING, AND FREDERICK M. AUSUBEL*
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, and Department of Molecular Biology,
Massachusetts General Hospital, Boston, Massachusetts 02114
Received 26 May 1989/Accepted 4 October 1989
To differentiate among the roles of the three nodD genes ofRhizobium meliloti 1021, we studied the activation
of a nodC-lacZ fusion by each of the three nodD genes in response to root exudates from several R. meliloti host
plants and in response to the flavone luteolin. We found (i) that the nodD, and nodD2 products (NodD, and
NodD2) responded differently to root exudates from a variety ofhosts, (ii) that NodDl but not NodD2 responded
to luteolin, (iii) that NodD2 functioned synergistically with NodD, or NodD3, (iv) that NodD2 interfered with
NodDl-mediated activation of nodC-lacZ in response to luteolin, and (v) that a region adjacent to and upstream
of nodD2 was required for NodD2-mediated activation of nodC-lacZ. We also studied the ability of each of the
three R. meliloti nodD genes to complement nodD mutations in R. trifolii and Rhizobium sp. strain NGR234. We
found (i) that nodD1 complemented an R. trifolii nodD mutation but not a Rhizobium sp. strain NGR234 nodD,
mutation and (ii) that R. meliloti nodD2 or nodD3 plus R. melioti syrM complemented the nodD mutations in

  

Source: Ausubel, Frederick M. - Department of Genetics, Harvard University

 

Collections: Biology and Medicine