Summary: be visualized as the N-MBDs acting as
a mechanical brake of the transporting
machinery by physically restricting the
rate-limiting movement.
The described model certainly leaves
several structural questions unanswered.
How, or where, does the Cu+
-loaded
chaperone interact with the ATPase?
Can the arrangement of transmembrane
segments be better defined? How are
multiple N-MBDs accommodated in the
structure? Interestingly, these issues are
within the reach of cryo-EM approaches.
As in the case of the role of N-MBDs,
addressing these would have a significant
impact in the field.
REFERENCES
Argu¨ ello, J.M., Eren, E., and Gonza´ lez-Guerrero,
M. (2007). Biometals 20, 233­248.

Source: Asbury, Chip - Department of Physiology and Biophysics, University of Washington at Seattle

Collections: Biology and Medicine