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Z .Journal of Immunological Methods 231 1999 93104 www.elsevier.nlrlocaterjim

Summary: Z .Journal of Immunological Methods 231 1999 93­104
z /Selectively infective phage SIP technology: scope and
Sabine Jung, Katja M. Arndt, Kristian M. Muller, Andreas Pluckthun )
¨ ¨
Biochemisches Institut, UniÕersitat Zurich, Winterthurerstr. 190, CH-8057 Zurich, Switzerland¨ ¨ ¨
Z .We review here the selectively infective phage SIP technology, a powerful tool for the rapid selection of protein­ligand
and peptide­ligand pairs with very high affinities. SIP is highly suitable for discriminating between molecules with subtle
stability and folding differences. We discuss the preferred types of applications for this technology and some pitfalls inherent
in the in vivo SIP method that have become apparent in its application with highly randomized libraries, as well as some
precautions that should be taken in successfully applying this technology. q 1999 Elsevier Science B.V. All rights reserved.
Keywords: Phage display; Affinity maturation; Disulfide bonds; ScFv fragments
1. Introduction
1.1. The principle of SIP
Z .The selectively infective phage SIP technology
was developed for selecting interacting protein­
Zligand pairs Duenas and Borrebaeck, 1994; Gra-~
.matikoff et al., 1994; Krebber et al., 1995 . It has


Source: Arndt, Katja - Institut für Biologie III, Albert-Ludwigs-Universität Freiburg
Müller, Kristian - Institut für Biologie III, Albert-Ludwigs-Universität Freiburg


Collections: Biology and Medicine; Biotechnology