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Vol. 59, No. 6INFECTION AND IMMUNITY, June 1991, p. 2023-2028 0019-9567/91/062023-06$02.00/0
 

Summary: Vol. 59, No. 6INFECTION AND IMMUNITY, June 1991, p. 2023-2028
0019-9567/91/062023-06$02.00/0
Copyright C 1991, American Society for Microbiology
Cloned Alpha and Beta C-Protein Antigens of Group B Streptococci
Elicit Protective Immunity
JAMES L. MICHEL,'* LAWRENCE C. MADOFF,"12 DAVID E. KLING,' DENNIS L. KASPER,12
AND FREDERICK M. AUSUBEL3
Channing Laboratory, Department ofMedicine, Brigham and Women's Hospital, 180 Longwood Avenue,1 and
Division ofInfectious Diseases, Beth Israel Hospital, Department ofMedicine, Harvard Medical
School,2 Boston, Massachusetts 02115, and Department ofMolecular Biology, Wellman-10,
Massachusetts General Hospital, Fruit Street, Boston, Massachusetts 021143
Received 7 January 1991/Accepted 27 February 1991
Streptococcus agalactiae (group B streptococci [GBS]) is the leading cause of neonatal sepsis and meningitis
in the United States. The surface-associated C proteins of GBS play a role in immunity, but their number, size,
structure, function, and virulence properties have not been well characterized. A recombinant library of DNA
fragments from GBS strain A909 (type Ia/C) was prepared in the plasmid pUX12, a specially constructed
Escherichia coli expression vector. The library was screened with a rabbit antiserum shown to be protective for
passive immunity to GBS infection in a mouse lethality model. Clones were divided into two distinct groups on
the basis of DNA-DNA cross-hybridization, restriction enzyme analysis, and the expression of antigenic
proteins in E. coli. A characteristic clone from each group was chosen for further study. Clone pJMS23

  

Source: Ausubel, Frederick M. - Department of Genetics, Harvard University

 

Collections: Biology and Medicine