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HORTSCIENCE 43(6):19221924. 2008. Morphogenetic Response of Lilium

Summary: HORTSCIENCE 43(6):1922­1924. 2008.
Morphogenetic Response of Lilium
michiganense to Four Auxin-type Plant
Growth Regulators In Vitro
James Robert Ault2
and Sandy S. Siqueira1
Chicago Botanic Garden, Ornamental Plant Research, 1000 Lake Cook
Road, Glencoe, IL 60022
Additional index words. tissue culture, propagation, callus induction, Michigan lily
Abstract. Shoot, root, and callus induction were examined in the North American lily
Lilium michiganense in response to treatment with four auxin-type plant growth
regulators (PGR). Seed from controlled crosses were aseptically excised from slightly
immature capsules and cultured in vitro on Murashige and Skoog basal medium and
vitamins with 30 gÁL­1
sucrose, 7.0 gÁL­1
agar, and a pH = 5.7. Seed were maintained at 20 8C
with a 14-h photoperiod. After 5.0 to 5.5 months, leaves and roots were removed from
seedlings, the bulbs transversely sectioned, then the bulb sections cultured cut-surface
down on the identical medium supplemented with 0.0, 1.0, 2.0, 4.0, or 8.0 mM dicamba,
picloram, K-NAA, or 2,4-D. Morphogenetic response was tabulated 4 months after


Source: Ault, James R. - Chicago Botanic Garden


Collections: Biology and Medicine