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Fourier analysis of light scattered by elongated scatterers Zeev Schiffer, Yosef Ashkenazy, Reuven Tirosh, and Mordechai Deutsch
 

Summary: Fourier analysis of light scattered by elongated scatterers
Zeev Schiffer, Yosef Ashkenazy, Reuven Tirosh, and Mordechai Deutsch
Biological stimulation of living cells is sometimes associated with morphological changes. A practical
method is developed to monitor cell stimulation by means of their conformational changes through
interpretation of the pattern of light scattered from a cell population. For this purpose a mathematical
model is suggested that predicts the power spectrum from a population of elliptic objects with a given
eccentricity. A computer simulation of that model is presented together with supporting experimental
results of the simulation. The predicted and the measured spectra are in good agreement. This
technique was applied to elongated cells that become circular on exposure to a human hormone, indi-
cating the potential applicability of the method in biology and medicine. The method and the apparatus
presented in this study could be applied to bioassays of cell systems that respond to a variety of stimulants
and to trace quantitatively the structural changes that occur during biological processes. 1999
Optical Society of America
OCIS codes: 000.1430, 070.4790, 100.3190, 170.1530, 260.1960, 290.5820.
1. Introduction
The analysis of light scattered by small round parti-
cles is a powerful tool for determining important pa-
rameters, such as size and structure, within a given
population. A very simple description of the scatter-
ing intensity is available13 when the suspending me-

  

Source: Ashkenazy, Yossi "Yosef" - Department of Solar Energy and Environmental Physics, Jacob Blaustein Institutes for Desert Research,Ben-Gurion University of the Negev

 

Collections: Physics; Environmental Management and Restoration Technologies