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TECHNOLOGY REPORT Identification of Immunological Reagents for Use in the

Identification of Immunological Reagents for Use in the
Study of Freshwater Planarians by Means of Whole-Mount
Immunofluorescence and Confocal Microscopy
Sofia M.C. Robb and Alejandro Sa´ nchez Alvarado*
University of Utah School of Medicine, Department of Neurobiology and Anatomy, Salt Lake City, Utah
Received 14 December 2001; Accepted 19 February 2002
Summary: In recent years, interest in planarians as a
model system for the study of metazoan regeneration,
adult stem cell biology, and the evolution of metazoan
body plans has been growing steadily. The availability of
RNA interference (RNAi), BrdU-labeling of planarian
stem cells, and thousands of planarian cDNA sequences
soon to be released into public databases has opened
planarians to molecular dissection. However, the suc-
cessful application of large-scale RNAi-based screens,
for example, will depend in part on the availability of
markers to characterize the resulting phenotypes. Given
the paucity of antibodies available for the study of pla-
narian biology, we have screened various public and


Source: Alvarado, Alejandro Sánchez - Department of Neurobiology and Anatomy, University of Utah


Collections: Biology and Medicine