Summary: Plant Molecular Biology 40: 167177, 1999.
© 1999 Kluwer Academic Publishers. Printed in the Netherlands.
Identification of a rice APETALA3 homologue by yeast two-hybrid
Yong-Hwan Moon, Ji-Young Jung, Hong-Gyu Kang and Gynheung An
Department of Life Science, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea
(author for correspondence)
Received 19 October 1998; accepted in revised form 6 January 1999
Key words: APETALA3, MADS gene, protein-protein interaction, rice, transcription activation, yeast two-hybrid
A cDNA clone OsMADS16 was isolated from the rice young inflorescence cDNA expression library by the yeast
two-hybrid screening method with OsMADS4 as bait. We have previously shown that the OsMADS4 gene is a
member of the PI family and that the MADS-box gene is involved in controlling development of the second and
third whorls of rice flowers. The sequence comparison indicated that OsMADS16 belongs to the AP3 family. The
OsMADS16 protein contains a PI-derived motif, FAFRVVPSQPNLH, that is a conserved sequence in AP3 family
genes at the C-terminal region. In addition, OsMADS16 contains a paleoAP3 motif, YGGNHDLRLG, downstream
of the PI-derived motif. The paleoAP3 motif is a consensus sequence in the C-terminal region of the AP3 family
genes of lower eudicot and magnolid dicot species. RNA blot analysis showed that the OsMADS16 gene was