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A quantitative method for assessing co-localization in immunolabeled thin section electron micrographs
 

Summary: A quantitative method for assessing co-localization in
immunolabeled thin section electron micrographs
James B. Anderson,a
Andrew A. Carol,b
Vanessa K. Brown,b
and Louise E. Andersonb,*
a
Department of Chemistry, Pennsylvania State University, University Park, PA 16802, USA
b
Department of Biological Sciences, University of Illinois-Chicago, 845 West Taylor, Chicago, IL 60607, USA
Received 31 March 2003, accepted 14 July 2003
Abstract
A method is introduced for the analysis of nearest neighbor distances between immunogold particles marking proteins on
electron micrographs. Deviation from the distribution that is predicted by chance indicates co-localization of the labeled species, and
the potential for productive interaction in vivo. Application of this method to the analysis of nearest neighbor distances in ex-
periments with pea leaf thin sections and isozyme-directed antibodies indicates that glyceraldehyde-3-P dehydrogenase is located
near P-glycerate kinase and near aldolase in the chloroplast stroma, consistent with the notion that these enzymes are part of a
multi-enzyme photosynthetic CO2-fixation complex in situ.
2003 Elsevier Inc. All rights reserved.
Keywords: Pisum sativum; Nearest neighbor analysis; Functional enzyme complexes; Co-localization; Glyceraldehyde-3-P dehydrogenase; Aldolase;

  

Source: Anderson, James B. - Department of Chemistry, Pennsylvania State University

 

Collections: Chemistry