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Immunisation of mice against neosporosis Anna Lundena,*, Stephen Wrightb

Summary: Immunisation of mice against neosporosis
Anna Lunde“na,*, Stephen Wrightb
, Judith E. Allenc
, David Buxtonb
Department of Parasitology (SWEPAR), Swedish University of Agricultural Sciences and National Veterinary Institute, SE-751 89 Uppsala, Sweden
Moredun Research Institute, Pentlands Science Park, Bush Loan by Edinburgh, Midlothian EH26 0PZ Scotland, UK
Institute of Cell, Animal and Population Biology, University of Edinburgh, West Mains Road, Edinburgh EH9 3JT, Scotland, UK
Received 14 December 2001; received in revised form 7 February 2002; accepted 13 February 2002
In the present study a murine encephalitis model was used to investigate if protection against neosporosis could be achieved by
immunisation. Groups of 10 mice were immunised with a sublethal dose of live Neospora caninum tachyzoites, N. caninum antigens
incorporated into iscoms, N. caninum lysate mixed with Quil A, or N. caninum lysate in PBS. Control mice were given Quil A only.
Challenge infection with 2.5 £ 106
N. caninum tachyzoites resulted in clinical symptoms that remained until the end of the experiment in the
controls. In contrast, mice immunised with live parasites or parasite lysate in Quil A only showed mild and transient symptoms. Of nine mice
immunised with N. caninum iscoms, seven recovered while two died. Most severely affected were the mice immunised with parasite lysate
only; all of them died within 28 days post-infection. Histological examination and scoring of brain lesions gave a significantly lower šP ,


Source: Allen, Judith - School of Biological Sciences, University of Edinburgh


Collections: Biology and Medicine