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Journal of Neuroscience Methods 140 (2004) 169181 Gene dose-dependent alterations in extraneuronal serotonin but not

Summary: Journal of Neuroscience Methods 140 (2004) 169181
Gene dose-dependent alterations in extraneuronal serotonin but not
dopamine in mice with reduced serotonin transporter expression
Tiffany A. Mathewsa, Denise E. Fedelea, Francesca M. Coppellia, Amy M. Avilac,
Dennis L. Murphyc, Anne M. Andrewsa,b,
a Department of Chemistry, The Pennsylvania State University, 104 Chemistry Building, University Park, PA 16802-6300, USA
b Huck Institute for Life Sciences, The Pennsylvania State University, University Park, PA 16802-6300, USA
c Laboratory of Clinical Science, National Institute of Mental Health, National Institutes of Health,
10 Center Drive MSC 1264, Bethesda, MD 20892-1264, USA
Received 10 February 2004; accepted 17 May 2004
Serotonin (5-HT) plays an integral regulatory role in mood, anxiety, cognition, appetite and aggressive behavior. Many therapeutic and
illicit drugs that modulate these functions act at the serotonin transporter (SERT), thus a mouse model with reduced transporter expression was
created to further investigate the effects of differential serotonin reuptake. In the present study, in vivo microdialysis was used to determine
homeostatic alterations in extracellular 5-HT levels in unanesthetized SERT knockout mice. SERT-/-
mice had significantly higher levels of
basal dialysate 5-HT than SERT+/+
mice in striatum and frontal cortex. In addition, although gene-specific increases in 5-HT were evident,
neuroadaptive alterations in dialysate dopamine levels were not detected in striatum. Zero net flux microdialysis was utilized to further
investigate alterations in extracellular 5-HT. Using this method, a gene dose-dependent increase in extraneuronal 5-HT was observed in


Source: Andrews, Anne M. - Huck Institutes of the Life Sciences, Pennsylvania State University


Collections: Biology and Medicine