Advanced Search

Browse by Discipline

Scientific Societies

E-print Alerts

Add E-prints

E-print Network

  Advanced Search  

Plant Biotechnology Journal (2007) 5, pp. 455464 doi: 10.1111/j.1467-7652.2007.00257.x 2007 The Authors

Summary: Plant Biotechnology Journal (2007) 5, pp. 455464 doi: 10.1111/j.1467-7652.2007.00257.x
2007 The Authors
Journal compilation 2007 Blackwell Publishing Ltd 455
Blackwell Publishing LtdOxford, UKPBIPlant Biotechnology Journal1467-7644 2007 Blackwell Publishing LtdXXXOriginal ArticlesBt Cry protein targets plant-parasitic nematodeXiang-Qian Li et al.
Resistance to root-knot nematode in tomato roots
expressing a nematicidal Bacillus thuringiensis
crystal protein
Xiang-Qian Li, Jun-Zhi Wei, Anderson Tan and Raffi V. Aroian*
Section of Cell and Developmental Biology, University of California, San Diego, La Jolla, CA 92093-0349, USA
Our laboratory has demonstrated previously that Bacillus thuringiensis (Bt) crystal (Cry)
proteins present in the Cry5 and Cry6 subclades intoxicate free-living nematodes. In this
study, we tested whether the expression of nematicidal Cry6A in transgenic plants provided
protection against plant-parasitic nematodes. As bacterial codon usage is incompatible with
expression in plants, two different codon-modified cry6A genes were synthesized for
expression in plants. One was designed by maintaining codon diversity whilst removing
codons not common in plants, and the other was designed by selecting the optimal codon
for each amino acid based on the Arabidopsis genome. Both versions of the cry6A gene,
driven by the constitutive cauliflower mosaic virus 35S promoter, were introduced into
tomato roots via Agrobacterium rhizogenes. Although both were found to express Cry6A


Source: Aroian, Raffi V. - Division of Biological Sciences, University of California at San Diego


Collections: Biology and Medicine