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Nanoliter-Volume 1 H NMR Detection Using Periodic

Summary: Nanoliter-Volume 1
H NMR Detection Using Periodic
Stopped-Flow Capillary Electrophoresis
Dean L. Olson,, Michael E. Lacey,,| Andrew G. Webb,,| and Jonathan V. Sweedler*,,|
Department of Chemistry, Electrical and Computer Engineering, and the Beckman Institute,
University of Illinois at Urbana-Champaign, Urbana, Illinois 61801
Recent advances in the analysis of nanoliter volumes using
1H NMR microcoils have led to the application of micro-
coils as detectors for capillary electrophoresis (CE).
Custom NMR probes consisting of 1-mm-long solenoidal
microcoils are fabricated from 50-m diameter wire
wrapped around capillaries to create nanoliter-volume
detection cells. For geometries in which the capillary and
static magnetic field are not parallel, the electrophoretic
current induces a magnetic field gradient which degrades
the spectroscopic information obtainable from CE/NMR.
To reduce this effect and allow longer analyte observation
times, the electrophoretic voltage is periodically inter-
rupted so that 1-min high-resolution NMR spectra are
obtained for every 15 s of applied voltage. The limits of


Source: Andrews, Anne M. - Huck Institutes of the Life Sciences, Pennsylvania State University


Collections: Biology and Medicine