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Molecular Ecology Notes (2001) 1, 293296 2001 Blackwell Science Ltd
 

Summary: Molecular Ecology Notes (2001) 1, 293296
2001 Blackwell Science Ltd
Blackwell Science, Ltd
PRIMER NOTE
Genetic variation in two populations of the rough-skinned
newt (Taricha granulosa) assessed using novel
tetranucleotide microsatellite loci
ADAM G. JONES, MICHAEL S. BLOUIN and STEVAN J. ARNOLD
Department of Zoology, 3029 Cordley Hall, Oregon State University, Corvallis, OR 97331, USA
Abstract
Seven novel tetranucleotide microsatellite loci were identified from a partial genomic DNA
library, enriched for GATA-motif microsatellites, from the rough-skinned newt (Taricha
granulosa). All loci were polymorphic, and one displayed a high frequency null allele. A
related species, T. rivularis, displays strong site fidelity and detectable population structure
over small spatial scales, so we assessed genetic variation in two samples of T. granulosa
separated by 16 km. Distributions of allele frequencies differ significantly between our
two sites, but small values of FST and RhoST suggest that the populations are linked by a
large amount of gene flow.
Keywords: homing, microsatellites, newts, parentage, population structure, site fidelity
Received 8 May 2001; revision accepted 4 July 2001

  

Source: Arnold, Stevan J. - Department of Zoology, Oregon State University
Jones, Adam - Department of Biology, Texas A&M University

 

Collections: Biology and Medicine; Environmental Sciences and Ecology