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Genes and Immunity (2001) 2, 1119 2001 Nature Publishing Group All rights reserved 1466-4879/01 $15.00
 

Summary: Genes and Immunity (2001) 2, 1119
2001 Nature Publishing Group All rights reserved 1466-4879/01 $15.00
www.nature.com/gene
Hepatitis B surface antigen- and tetanus toxoid-specific
clonal expansion of CD4+
cells in vitro determined by
TCRBV CDR3 length and nucleotide sequence
GP Uko, PA Fraser, ZL Awdeh, DA Fici, KD Crawford, CE Larsen and CA Alper
The Center for Blood Research and the Departments of Medicine and Pediatrics, Harvard Medical School, Boston, MA 02115, USA
We demonstrate activation of primary human TCRBV-specific CD4+
cells in vitro towards hepatitis B surface antigen
(HBsAg) and tetanus toxoid (TT) without the use of cell lines, clones or added cytokines. By multiplex PCR analysis and
spectratyping, antigen-activated cells exhibited clonal T cell receptor expansion within specific and limited TCRBV families.
The expanded CD4+
T cells were CD45RO+
. Three of four unrelated HBsAg responders showed CD4+
expansion within
the TCRBV16 family. The response comprised predominantly single CDR3 sequences in all three donors and was
completely monoclonal in one of them. However, the CDR3 lengths and sequences differed among the responders.
Clonality induced by HBsAg in TCRBV16 was specific, reproducible and distinct from that induced by TT in terms of

  

Source: Alper, Chester A. - Immune Disease Institute, Harvard University

 

Collections: Biology and Medicine