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Molecular Ecology Notes (2003) 3, 180182 doi: 10.1046/j.1471-8286.2003.00390.x 2003 Blackwell Publishing Ltd
 

Summary: Molecular Ecology Notes (2003) 3, 180­182 doi: 10.1046/j.1471-8286.2003.00390.x
© 2003 Blackwell Publishing Ltd
Blackwell Publishing Ltd.
PRIMER NOTE
Isolation and cross-species amplification of microsatellite
loci from the fucoid seaweeds Fucus vesiculosus, F. serratus
and Ascophyllum nodosum (Heterokontophyta, Fucaceae)
C. R. ENGEL,*§ S. H. BRAWLEY, K. J. EDWARDS and E. SERRÃO*
*Centro de Ciencias do Mar, FCMA, Universidade do Algarve, 8005­139 Faro, Portugal, School of Marine Sciences, 5722 Deering
Hall, University of Maine, Orono, ME 04469­5722, USA, School of Biological Sciences, University of Bristol, Bristol, BS8 1UG, UK
Abstract
The Fucaceae is a family of brown seaweeds that dominate and frequently co-occur on
North Atlantic rocky shores. We developed nine polymorphic microsatellite markers for
the fucoid seaweeds Fucus vesiculosus, F. serratus and Ascophyllum nodosum using a com-
bined, enriched library. Six of these loci were polymorphic in at least two species, showing
from two to eight alleles with heterozygosities ranging from 0.41 to 0.85. Loci were also
tested on F. spiralis, revealing five polymorphic microsatellite loci in this species.
Keywords: cross-species amplification, Fucaceae, microsatellites, seaweeds
Received 15 October 2002; revision received 18 December 2002; accepted 18 December 2002
The Fucaceae (Phaeophyceae) are a family of closely

  

Source: Alberto, Filipe - Centro de Ciencias do Mar, Universidade do Algarve

 

Collections: Environmental Sciences and Ecology; Biology and Medicine