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Rice SCAMP1 Defines Clathrin-Coated, trans-GolgiLocated Tubular-Vesicular Structures as an Early Endosome

Summary: Rice SCAMP1 Defines Clathrin-Coated, trans-Golgi­Located
Tubular-Vesicular Structures as an Early Endosome
in Tobacco BY-2 Cells W OA
Sheung Kwan Lam,a Ching Lung Siu,a Stefan Hillmer,b Seonghoe Jang,c Gynheung An,c David G. Robinson,b
and Liwen Jianga,1
a Department of Biology and Molecular Biotechnology Program, Chinese University of Hong Kong, Shatin, New Territories,
Hong Kong, China
b Department of Cell Biology, Heidelberg Institute for Plant Sciences, University of Heidelberg, D-69120 Heidelberg, Germany
c National Research Laboratory of Plant Functional Genomics, Division of Molecular and Life Sciences, Pohang
University of Science and Technology, Pohang 790-784, Korea
We recently identified multivesicular bodies (MVBs) as prevacuolar compartments (PVCs) in the secretory and endocytic
pathways to the lytic vacuole in tobacco (Nicotiana tabacum) BY-2 cells. Secretory carrier membrane proteins (SCAMPs) are
post-Golgi, integral membrane proteins mediating endocytosis in animal cells. To define the endocytic pathway in plants, we
cloned the rice (Oryza sativa) homolog of animal SCAMP1 and generated transgenic tobacco BY-2 cells expressing yellow
fluorescent protein (YFP)­SCAMP1 or SCAMP1-YFP fusions. Confocal immunofluorescence and immunogold electron
microscopy studies demonstrated that YFP-SCAMP1 fusions and native SCAMP1 localize to the plasma membrane and mobile
structures in the cytoplasm of transgenic BY-2 cells. Drug treatments and confocal immunofluorescence studies demon-
strated that the punctate cytosolic organelles labeled by YFP-SCAMP1 or SCAMP1 were distinct from the Golgi apparatus and
PVCs. SCAMP1-labeled organelles may represent an early endosome because the internalized endocytic markers FM4-64 and
AM4-64 reached these organelles before PVCs. In addition, wortmannin caused the redistribution of SCAMP1 from the early


Source: An, Gynheung - Department of Life Science, Pohang University of Science and Technology


Collections: Biotechnology; Biology and Medicine