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Summary: 1
Molecular Cell, Volume 41
Supplemental Information
Robust Control of Nitrogen Assimilation
by a Bifunctional Enzyme in E. coli
Yuval Hart, Daniel Madar, Jie Yuan, Anat Bren, Avraham E. Mayo,
Joshua D. Rabinowitz, and Uri Alon
Contents:
1. Metabolite levels at different GS induction levels....................2
2. Growth rate is robust to variations in AT/AR levels ................2
3. Robustness breaks down under the expression of a mono-functional mutant of the
AT/AR enzyme in a strain deleted for AT/AR.............................4
4. Under nitrogen limiting conditions robustness to GS levels is lost and the GS
encoding gene is transcriptionally activated.................................6
5. Mathematical models of the GS system with no avidity do not display robustness 6
6. Strains and plasmids used in this study.....................................9
7. Methods for strain and plasmid constructions and growth rate measurements 9
8. Calculation of fold induction of pTrc99A-derived plasmids....11
9. Measurements of glnA promoter activity..................................12
10. Analytical solution for the binding of AT to one and two GS subunits 12
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