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Precision of Interferometric Diffusion Coefficients in a Four-Component System Relevant to Protein Crystal Growth: Lysozyme-Tetra(ethylene glycol)-NaCl-H2O
 

Summary: Precision of Interferometric Diffusion Coefficients in a Four-Component System Relevant to
Protein Crystal Growth: Lysozyme-Tetra(ethylene glycol)-NaCl-H2O
Onofrio Annunziata, Alessandro Vergara, Luigi Paduano, Roberto Sartorio,*,
Donald G. Miller, and John G. Albright#
Massachusetts Institute of Technology, Cambridge, Massachusetts, Chemistry Department, UniVersity
"Federico II" of Naples, Via Cinthia 80126 Naples, Italy, Lawrence LiVermore National Laboratory,
LiVermore L-203, California 94550, and Texas Christian UniVersity, Fort Worth, Texas 76129
ReceiVed: January 27, 2003; In Final Form: March 31, 2003
We investigate the precision of Rayleigh interferometry in evaluating the nine mutual diffusion coefficients
Dij defining a four-component system by investigating the system lysozyme-tetra(ethylene glycol)-NaCl-
H2O. We believe our results to be the most successful experimental determination of diffusion coefficients in
quaternary systems. This choice of system is motivated by its relevance to protein crystal growth. The
comparison of the Dij coefficients of the quaternary system and the Dij coefficients from the corresponding
ternary and binary systems enables us to extract some information about the hydrodynamics and
thermodynamics in solutions where protein crystals grow.
I. Introduction
Optical interferometry provides the most precise methods for
determining mutual diffusion coefficients in liquids.
The principal purpose of this methodological paper is to test
the precision of diffusion coefficient measurements by Rayleigh

  

Source: Annunziata, Onofrio - Department of Chemistry, Texas Christian University

 

Collections: Chemistry