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Insect Biochemistry and Molecular Biology 31 (2001) 553562 www.elsevier.com/locate/ibmb

Summary: Insect Biochemistry and Molecular Biology 31 (2001) 553562
Cloning and sequence of the gene encoding the muscle fatty acid
binding protein from the desert locust, Schistocerca gregaria
Qiwei Wu, Peter Andolfatto 1
, Norbert H. Haunerland *
Department of Biological Sciences, Simon Fraser University, Burnaby, BC, Canada V5A 1S6
Received 25 May 2000; received in revised form 18 September 2000; accepted 18 September 2000
Muscle fatty acid binding protein (FABP) is a major cytosolic protein in flight muscle of the desert locust, Schistocerca gregaria.
FABP expression varies greatly during development and periods of increased fatty acid utilization, but the molecular mechanisms
that regulate its expression are not known. In this study, the gene coding for locust muscle FABP was amplified by PCR and
cloned, together with 1.2 kb of upstream sequence. The sequence coding for the 607 bp cDNA is interrupted by two introns of
12.7 and 2.9 kb, inserted in analogous positions as the first and third intron of the mammalian homologues. Both introns contain
repetitive sequences also found in other locust genes, and the second intron contains a GT-microsatellite. The promoter sequence
includes a canonical TATA box 24 bp upstream of the transcription start site. The upstream sequence contains various potential
myocyte enhancer sequences and a 160 bp segment that is repeated three times. In database searches in the genome database of
Drosophila melanogaster, a gene with the same gene organization and promoter structure was identified, likely the dipteran homol-
ogue of muscle FABP. Upstream of both insect genes, a conserved 19 bp inverted repeat sequence was detected. A similar but
reverse palindrome is also present upstream of all mammalian heart FABP genes, possibly representing a novel element involved


Source: Andolfatto, Peter - Department of Ecology and Evolutionary Biology, Princeton University
Haunerland, Norbert H. - Department of Biological Sciences, Simon Fraser University


Collections: Biology and Medicine