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Plant Physiol. (1987) 83, 15-23 0032-0889/87/83/0015/09/$01.00/0
 

Summary: Plant Physiol. (1987) 83, 15-23
0032-0889/87/83/0015/09/$01.00/0
Gibberellin-Induced Changes in the Populations of Translatable
mRNAs and Accumulated Polypeptides in Dwarfs of Maize and
Pea'
Received for publication June 23, 1986 and in revised form September 4, 1986
JOANNE CHORY, DANIEL F. VOYTAS, NEIL E. OLSZEWSKI, AND FREDERICK M. AUSUBEL*
Department ofGenetics, Harvard Medical School, and Department ofMolecular Biology, Massachusetts
General Hospital, Boston, Massachusetts 02114
ABSTRACI
Two-dimensional gel electrophoresis was used to characterize the
molecular mechanism of gibberellin-induced stem elongation in maize
and pea. Dwarf mutants of maize (d-5) and pea (Progress No. 9) lack
endogenous gibberellin (GA,) but become phenotypically normal with
exogenous applications of this hormone. Sections from either etiolated
maize or green pea seedlings were incubated in the presence of [35S]
methionine for 3 hours with or without gibberellin. Labeled proteins from
soluble and particulate fractions were analyzed by two-dimensional gel
electrophoresis and specific changes in the patterns of protein synthesis
were observed upon treatment with gibberellin. Polyadenylated mRNAs

  

Source: Ausubel, Frederick M. - Department of Genetics, Harvard University

 

Collections: Biology and Medicine