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Toward Fully Automated High-Resolution Electron Tomography JENNIFER C. FUNG
 

Summary: Toward Fully Automated High-Resolution Electron Tomography
JENNIFER C. FUNG
Graduate Group in Biophysics, University of California at San Francisco, San Francisco, CA 94143-0448
AND
WEIPING LIU, W. J. DE RUIJTER, HANS CHEN, CRAIG K. ABBEY, JOHN W. SEDAT, AND DAVID A. AGARD
1
Department of Biochemistry and Biophysics and Howard Hughes Medical Institute, University of California at San Francisco,
San Francisco, CA 94143-0448
Received June 2, 1995, and in revised form August 3, 1995
Electron tomography is a powerful tool in eluci-
dating the three-dimensional architecture of large
biological complexes and subcellular organelles. Its
use can be expanded through the simplification of
the tomographic procedure by automation of its
tasks. In this paper, we describe our EMACT/
EMCAT system, which automates both tomographic
data collection and reconstruction. 1996 Academic
Press, Inc.
INTRODUCTION
In 1968, the bacteriophage T4 tail was recon-

  

Source: Agard, David - Department of Biochemistry and Biophysics, University of California at San Francisco

 

Collections: Biotechnology; Biology and Medicine