Advanced Search

Browse by Discipline

Scientific Societies

E-print Alerts

Add E-prints

E-print Network

  Advanced Search  

Plant Science 167 (2004) 417426 Stress-inducible OsP5CS2 gene is essential for salt and

Summary: Plant Science 167 (2004) 417426
Stress-inducible OsP5CS2 gene is essential for salt and
cold tolerance in rice
Junghe Hura,b,1, Ki-Hong Junga,1, Choon-Hwan Leeb, Gynheung Ana,
a National Research Laboratory of Plant Functional Genomics, Division of Molecular and Life Sciences,
Pohang University of Science and Technology (POSTECH), Pohang 790-784, Republic of Korea
b Department of Molecular Biology, Pusan National University, Pusan 609-735, Republic of Korea
Received 29 January 2004; received in revised form 13 April 2004; accepted 14 April 2004
Available online 13 May 2004
We isolated a rice T-DNA tagging line, in which T-DNA was inserted into the sixth intron of OsP5CS2. This gene encodes for a protein that is
highly homologous to 1
-pyrroline-5-carboxylate synthetase (P5CS), a proline biosynthesis enzyme. The T-DNA contained the promoterless
gus gene, allowing generation of a gene fusion between OsP5CS2 and gus. Therefore, the expression pattern of OsP5CS2 could be easily
monitored by in situ GUS assay. At the seedling stage, the transcript level was low. However, gene expression was preferentially induced in the
dividing zone of the roots by salt, cold, or ABA treatments. In mature spikelets, the gene was expressed mainly in stamens. RT-PCR analyses
confirmed the results from the GUS assay. OsP5CS2 transcript was present in reproductive organs, especially the stamens. In seedling roots,
transcript levels were increased by treatment with 250 mM NaCl, 4
C cold stress, or 0.5 M ABA. Our OsP5CS2 knockout plants were more
sensitive to salt and cold stresses than were the wild-type controls. Root and shoot growth in the knockout seedlings were severely retarded


Source: An, Gynheung - Department of Life Science, Pohang University of Science and Technology


Collections: Biotechnology; Biology and Medicine