Home

About

Advanced Search

Browse by Discipline

Scientific Societies

E-print Alerts

Add E-prints

E-print Network
FAQHELPSITE MAPCONTACT US


  Advanced Search  

 
Cell, Vol. 117, 689691, June 11, 2004, Copyright 2004 by Cell Press start with a transcriptional regulatory network with aFlagellar Biosynthesis In Silico
 

Summary: Cell, Vol. 117, 689691, June 11, 2004, Copyright 2004 by Cell Press
Previews
start with a transcriptional regulatory network with aFlagellar Biosynthesis In Silico:
known connectivity diagram and to find both the func-
Building Quantitative Models tions integrating the regulatory inputs at each promoter
(Setty et al., 2003) and the parameters describing quanti-of Regulatory Networks
tatively how all the transcription factors regulate each
individual target promoter (Kalir et al., 2001; Ronen et
al., 2002). A parallelized GFP reporter gene assay was
In this issue of Cell, Kalir and Alon (2004) describe the first used to accurately measure the time courses of
construction of an in silico model for the regulatory promoter activity of flagellar biosynthetic genes in re-
network responsible for the control of flagellar biosyn- sponse to induction of this system. Second, the inte-
thesis in E. coli based on quantitative gene expression grated action of the two main regulators in the system,
data. They show how the model can be used as a FlhDC and FliA, was determined by constructing a strain
quantitative blueprint to design genetic modifications where these genes are expressed from independently
tunable promoters and measuring the activity of a singlewith predictable influence on the dynamics of the sys-
target promoter. This approach was used in an earliertem. The work by Kalir and Alon (2004) provides a
study of the classical lac regulatory system (Setty et al.,general approach for building detailed models of tran-
2003), but in the current case the integration functionscriptional regulatory networks.
was found to be a linear combination of the two inputs

  

Source: Alon, Uri - Departments of Molecular Cell Biology & Physics of Complex Systems, Weizmann Institute of Science

 

Collections: Biology and Medicine