Summary: Transgenic and Gene Targeting Core
9500 Gilman Drive, MC 0687
La Jolla, CA 920930687
Current as of Aug. 2008
TRANSGENIC MICE: METHOD FOR PREPARING DNA
(Courtesy of Rosenfeld lab members, UCSD)
THIS METHOD STRONGLY RECOMMENDED BY THE CORE
1. Grow your plasmid in a bacterial strain like XL1 Blue (Stratagene) that has no endoA
activity and does not produce a lot of carbohydrates. Grow a 250500 ml culture from
a fresh transformation.
2. Make a Qiagen500 DNA preparation according to the manufacturer's instructions.
Resuspend DNA in 500 ul TE.
3. Further purify the DNA obtained from the Qiagen preparation over a CsCl gradient, or
use the Promega Midi prep kit called "Pure yield Plasmic Midiprep." Catalog #A2492