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Transgenic and Gene Targeting Core 9500 Gilman Drive, MC 0687
 

Summary: Transgenic and Gene Targeting Core
9500 Gilman Drive, MC 0687
La Jolla, CA 920930687
(858) 5343178
Current as of Aug. 2008

TRANSGENIC MICE: METHOD FOR PREPARING DNA
FOR MICROINJECTION
(Courtesy of Rosenfeld lab members, UCSD)
THIS METHOD STRONGLY RECOMMENDED BY THE CORE

1. Grow your plasmid in a bacterial strain like XL1 Blue (Stratagene) that has no endoA
activity and does not produce a lot of carbohydrates. Grow a 250500 ml culture from
a fresh transformation.

2. Make a Qiagen500 DNA preparation according to the manufacturer's instructions.
Resuspend DNA in 500 ul TE.

3. Further purify the DNA obtained from the Qiagen preparation over a CsCl gradient, or
use the Promega Midi prep kit called "Pure yield Plasmic Midiprep." Catalog #A2492

  

Source: Abagyan, Ruben - School of Pharmacy and Pharmaceutical Sciences, University of California at San Diego

 

Collections: Biology and Medicine