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Summary: Identification of the ADP-glucose pyrophosphorylase isoforms
essential for starch synthesis in the leaf and seed endosperm
of rice (Oryza sativa L.)
Sang-Kyu Lee Æ Seon-Kap Hwang Æ Muho Han Æ Joon-Seob Eom Æ Hong-Gyu Kang Æ
Yulyi Han Æ Sang-Bong Choi Æ Man-Ho Cho Æ Seong Hee Bhoo Æ Gynheung An Æ
Tae-Ryong Hahn Æ Thomas W. Okita Æ Jong-Seong Jeon
Received: 9 December 2006 / Accepted: 17 February 2007 / Published online: 4 April 2007
Ó Springer Science+Business Media B.V. 2007
Abstract ADP-glucose pyrophosphorylase (AGP) cata-
lyzes the first committed step of starch biosynthesis in
higher plants. To identify AGP isoforms essential for this
biosynthetic process in sink and source tissues of rice
plants, we analyzed the rice AGP gene family which con-
sists of two genes, OsAGPS1 and OsAGPS2, encoding
small subunits (SSU) and four genes, OsAGPL1, OsA-
GPL2, OsAGPL3 and OsAGPL4, encoding large subunits
(LSU) of this enzyme heterotetrameric complex. Subcel-
lular localization studies using green fluorescent protein
(GFP) fusion constructs indicate that OsAGPS2a, the
product of the leaf-preferential transcript of OsAGPS2, and
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