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Comparison of Morpholino Based Translational Inhibition During the Development of Xenopus laevis and
 

Summary: Comparison of Morpholino Based Translational Inhibition
During the Development of Xenopus laevis and
Xenopus tropicalis
Stephen L. Nutt, Odile J. Bronchain, Katharine O. Hartley, and Enrique Amaya*
Wellcome/CRC Institute and Department of Zoology, University of Cambridge, Cambridge, United Kingdom
Received 26 March 2000; Accepted 22 April 2001
Published online 23 July 2001; DOI 10.1002/gene.1042
Summary: Morpholino (MO) based inhibition of transla-
tional initiation represents an attractive methodology to
eliminate gene function during Xenopus development
(Heasman et al., 2000). However, the degree to which a
given target protein can be eliminated and the longevity
of this effect during embryogenesis has not been docu-
mented. To examine the efficacy of MOs, we have used
transgenic Xenopus lines that harbour known numbers
of integrations of a GFP reporter under the control of the
ubiquitous and highly expressed CMV promoter (Fig. 1a).
In addition we have investigated the longevity of the
inhibitory effect by using transgenic lines expressing
GFP specifically in the lens of tadpoles. These trans-

  

Source: Amaya, Enrique - Healing Foundation Centre & Developmental Biology, Faculty of Life Sciences, University of Manchester

 

Collections: Biology and Medicine